Team:Warsaw/Calendar-Main/5 July 2008

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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
<p>'''Preparation of constructs with OmpA protein fusions'''<br>
1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) <br>  
1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega) <br>  
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2. </p>
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2. Control digest of plasmids with NotI and BamHI<br>
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3. Gel electrophoresis <br>
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</p>

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Preparation of constructs with OmpA protein fusions
1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)
2. Control digest of plasmids with NotI and BamHI
3. Gel electrophoresis