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Team:Warsaw/Calendar-Main/2 July 2008
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<p>'''Preparation of constructs with OmpA protein fusions'''<br> | <p>'''Preparation of constructs with OmpA protein fusions'''<br> | ||
| - | 1. PCR on OmpA_linker and linker_alpha with | + | 1. PCR on OmpA_linker and linker_alpha with |
| - | 2. PCR on OmpA_linker and linker_omega | + | <html> |
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a> | ||
| + | </html> | ||
| + | primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)<br> | ||
| + | 2. PCR on OmpA_linker and linker_omega with | ||
| + | <html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a> | ||
| + | </html> | ||
| + | primers (20 cycles, annealing length 1.15 min, annealing temperature 57 degrees)<br> | ||
3. Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega <br> | 3. Gel electrophoresis of PCR products and gel-out of OmpA_alpha and OmpA_omega <br> | ||
4. Overnight digestation of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI <br> | 4. Overnight digestation of purified OmpA_alpha and OmpA_omega DNA with NdeI and BamHI <br> | ||
Revision as of 14:31, 26 September 2008
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Preparation of constructs with OmpA protein fusions
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