Team:Chiba/jk/β/weak 1

From 2008.igem.org

(Difference between revisions)
(→Week 1)
Line 1: Line 1:
-
>[[Team:Chiba/jk/β|送受信班]]
+
>[[Team:Chiba/jk/γ|出力班]]
==Week 1==
==Week 1==
-
===17 August, 2008===
 
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:competent cells : XL10G
 
-
::*BBa_R0040(2008) [http://partsregistry.org/Part:BBa_R0040] -> No colonies on plates
 
-
::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] -> No colonies on plates
 
-
::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
 
-
::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
 
-
::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
 
-
::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] -> No colonies on plates
 
-
===18 August, 2008===
+
==17,August 2008==
-
:'''[[Team:Chiba/protocol/PCR|PCR]]'''
+
<br>PCR
-
::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070]
+
<br>BBa_E0430①
-
::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076]
+
<br>BBa_E0420②
-
::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077]
+
<br>BBa_J06702③
-
::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078]
+
<br>BBa_S01416④
-
::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079]
+
<br>BBa_I73003⑤
-
::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
+
<br>BBa_I73004⑥
-
 
+
<br>BBa_I730026⑦
-
 
+
<br>BBa_I763002⑧
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
-
<tr>
+
-
<td width="257">DNA Template</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP mix</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Foward Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Reverse Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA polymerase</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermopol Buffer</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>68</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>100μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
 
+
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+
-
 
+
-
 
+
-
 
+
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+
-
 
+
-
 
+
-
{|align="justify"
+
-
|[[Image:Chiba-0818.JPG]]
+
-
|
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample DNA</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
:From left;
+
-
::*BBa_C0070(2008) [http://partsregistry.org/Part:BBa_C0070] ->None
+
-
::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] ->None
+
-
::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] ->None
+
-
::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] ->None
+
-
::*BBa_C0079(2008) [http://partsregistry.org/Part:BBa_C0079] ->None
+
-
::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control ->OK
+
-
|}
+
-
 
+
-
 
+
-
 
+
-
 
+
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
+
-
competent cells : XL10G
+
-
 
+
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+
-
::*BBa_C0076(2006) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates(19/8)
+
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+
-
 
+
-
 
+
-
:--->(20/8)[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]
+
-
 
+
-
===19 August, 2008===
+
-
:'''[[Team:Chiba/protocol/PCR|PCR]]'''
+
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
+
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
+
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
+
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
+
-
::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154]
+
-
::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154]
+
-
::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control
+
-
 
+
-
 
+
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">DNA Template</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP mix</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Foward Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Reverse Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA polymerase VENT</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermopol Buffer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>28</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>50μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
 
+
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
+
-
 
+
-
 
+
-
 
+
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+
-
{|align="justify"
+
-
|[[Image:Chiba-0819.JPG]]
+
-
|
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample DNA</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
:From left;
+
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] ->None
+
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] ->None
+
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] ->None
+
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] ->None
+
-
::*BBa_S3154(2007) [http://partsregistry.org/Part:BBa_S3154] ->None
+
-
::*BBa_S3154(2006) [http://partsregistry.org/Part:BBa_S3154] ->None
+
-
::*BBa_F2620(2007) [http://partsregistry.org/Part:BBa_F2620]･･･control  ->OK
+
-
|}
+
-
 
+
-
 
+
-
 
+
-
===20 August, 2008===
+
-
:(18/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
+
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
+
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
+
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
+
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
+
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+
-
 
+
-
 
+
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+
-
{|align="justify"
+
-
|[[Image:Chiba-0820-3.JPG]]
+
-
|
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample DNA</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
:From left;
+
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
+
-
|}
+
-
 
+
-
 
+
-
 
+
-
{|align="justify"
+
-
|[[Image:Chiba-0820-4.JPG]]
+
-
|
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample DNA</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>4</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>24μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
:From left;
+
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
+
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> OK
+
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
+
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
+
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
+
-
|}
+
-
 
+
-
 
+
-
 
+
-
 
+
-
{|align="justify"
+
-
|[[Image:Chiba-0820.JPG]]
+
-
|
+
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
-
<tr>
+
-
<td width="257">Sample DNA</td>
+
-
<td>3</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12μL</td>
+
-
</tr>
+
-
</table>
+
-
 
+
-
:From left;<br>
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK<br>
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK<br>
+
-
|}
+
-
 
+
-
 
+
-
--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]
+
-
 
+
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
<tr>
<tr>
<td width="257">Sample No</td>
<td width="257">Sample No</td>
-
<td>1</td><td>2</td><td>3</td><td>4</td><td>5</td><td>6</td><td>7</td><td>8</td>
+
<td>①</td><td>②</td><td>③</td><td>④</td><td>⑤</td><td>⑥</td><td>⑦</td><td>⑧</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170]</td>
+
<td>DNA tamplate</td>
-
<td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
+
<td>1</td><td>1</td><td>1</td><td>1</td>><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170]</td>
+
<td>FW primer</td>
-
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td>
+
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178]</td>
+
<td>VR primer</td>
-
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td>
+
<td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
</tr>
</tr>
<tr>
<tr>
-
<td>BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178]</td>
+
<td>dNTPmix</td>
-
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>3</td>
+
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>EcoRⅠ</td>
+
<td>thermo pol buffer</td>
-
<td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
</tr>
</tr>
<tr>
<tr>
-
<td>PstⅠ</td>
+
<td>DNA pol(VENT)</td>
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Buffer EcoRⅠ</td>
+
-
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>BSA</td>
+
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
<td>dH2O</td>
<td>dH2O</td>
-
<td>4.9</td><td>4.9</td><td>4.9</td><td>4.9</td><td>4.8</td><td>4.8</td><td>4.8</td><td>4.8</td>
+
<td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td><td>68</td>
</tr>
</tr>
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
+
<td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td><td>100</td>
</tr>
</tr>
</table>
</table>
Line 365: Line 55:
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
+
==20,August 2008==
-
{|align="justify"
+
<br>TRANSFORMATION
-
|[[Image:Chiba-0820-2.JPG]]
+
<br>BBa_J52008(Luciferase Renilla)-->colony
-
|
+
<br>BBa_I712019(Luciferase Fire fly)-->no colony
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
<br>BBa_J332002(LacZ)-->colony
-
<tr>
+
<br>BBa_E2030(Venus YFP)-->not so good
-
<td width="257">Sample DNA</td>
+
<br>YFP(P-GEX)-->colony
-
<td>3</td>
+
<br>BBa_F2620-->colony
-
</tr>
+
<br>BBa_T9002-->colony
-
<tr>
+
-
<td>Loading Dye</td>
+
-
<td>2</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>7</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>12μL</td>
+
-
</tr>
+
-
</table>
+
-
:From left;
+
-
:(Single Digestion 1~4)
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
-
:(Double Digestion 1~4)
+
-
::*BBa_C0170(2006) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
-
::*BBa_C0170(2007) [http://partsregistry.org/Part:BBa_C0170] -> OK
+
-
::*BBa_C0178(2006) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
-
::*BBa_C0178(2007) [http://partsregistry.org/Part:BBa_C0178] -> OK
+
-
|}
+
-
===21 August, 2008===
 
-
:(20/8)--->'''[[Team:Chiba/protocol/PCR|PCR]]'''
 
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070]
 
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070]
 
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076]
 
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078]
 
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078]
 
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
+
==21,August 2008==
-
<tr>
+
-
<td width="257">DNA Template</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dNTP mix</td>
+
-
<td>10</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Foward Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Reverse Primer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>DNA polymerase TAQ</td>
+
-
<td>1</td>
+
-
</tr>
+
-
<tr>
+
-
<td>Thermopol Buffer</td>
+
-
<td>5</td>
+
-
</tr>
+
-
<tr>
+
-
<td>dH2O</td>
+
-
<td>28</td>
+
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>50μL</td>
+
-
</tr>
+
-
</table>
+
 +
<br>TRANSFORMATION
 +
<br>BBa_J32007(Lux CDABE)-->no colony
 +
<br>BBa_B0034(RBS)-->colony
-
:95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
 
 +
== 22,August 2008 ==
 +
<br>MINI PREP
 +
<br>BBa_J52008(Luciferase)
 +
<br>BBa_F2620
 +
<br>BBa_T9002
 +
<br>BBa_J33202(LacZ)
 +
<br>PUC19
 +
<br>PGEX VENUS YFP(GST fusion)
 +
<br>
 +
<br>TRANSFORMATION
 +
<br>PGEX VENUS YFP(GST fusion)
-
:--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0821.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample DNA</td>
 
-
<td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12μL</td>
 
-
</tr>
 
-
</table>
 
-
 
-
:From left;
 
-
::*BBa_C0078(2007) [http://partsregistry.org/Part:BBa_C0078] -> None
 
-
::*BBa_C0078(2006) [http://partsregistry.org/Part:BBa_C0078] -> None
 
-
::*BBa_C0076(2007) [http://partsregistry.org/Part:BBa_C0076] -> None
 
-
::*BBa_C0070(2007) [http://partsregistry.org/Part:BBa_C0070] -> OK
 
-
::*BBa_C0070(2006) [http://partsregistry.org/Part:BBa_C0070] -> OK
 
-
|}
 
-
 
-
 
-
 
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:competent cells : XL10G
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
 
-
 
-
 
-
 
-
 
-
--->(23/8)'''[[Team:Chiba/protocol/digestion|Digestion]]'''
 
-
 
-
 
-
 
-
===22 August, 2008===
 
-
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 
-
:competent cells : XL10G
 
-
::*BBa_J13002(2007) [http://partsregistry.org/Part:BBa_J13002]
 
-
::*BBa_J13002(2006) [http://partsregistry.org/Part:BBa_J13002]
 
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
 
-
::*BBa_J04500(2006) [http://partsregistry.org/Part:BBa_J04500]
 
-
:(Locate & 2 Punch ~iyama ver.~)
 
-
::*BBa_C0076(2008) [http://partsregistry.org/Part:BBa_C0076] -> No colonies on plates
 
-
::*BBa_C0077(2008) [http://partsregistry.org/Part:BBa_C0077] -> No colonies on plates
 
-
::*BBa_C0078(2008) [http://partsregistry.org/Part:BBa_C0078] -> No colonies on plates
 
-
::*BBa_J04500(2008) [http://partsregistry.org/Part:BBa_J04500] -> No colonies on plates
 
-
 
-
 
-
 
-
--->(24/8)'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
 
-
===23 August, 2008===
 
-
:(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
 
-
 
-
 
-
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
 
-
 
-
 
-
:<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="270">Sample No</td>
 
-
<td> 1</td><td> 2</td><td> 3</td><td> 4</td><td> 5</td><td> 6</td><td> 7</td><td> 8</td><td> 9</td><td>10</td><td>11</td><td>12</td><td>13</td><td>14</td><td>15</td><td>16</td><td>17</td><td>18</td><td>19</td><td>20</td><td>21</td>
 
-
</tr><tr>
 
-
<td>BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]</td>
 
-
<td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]</td>
 
-
<td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]</td>
 
-
<td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<tr>
 
-
<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td></tr>
 
-
<tr>
 
-
<td>BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td></tr>
 
-
<tr>
 
-
<td>BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>3</td></tr>
 
-
<tr>
 
-
<td>EcoRⅠ</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>PstⅠ</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Buffer EcoRⅠ</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.9</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td><td>0.8</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>-</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>3</td><td>1</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td><td>10</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
 
-
:--->[[Team:Chiba/protocol/gelcheck|Gel Check]]
 
-
{|align="justify"
 
-
|[[Image:Chiba-0823-1.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>1~6</td><td>7</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>2</td><td>4</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td><td>6</td>
 
-
</tr>
 
-
</table>
 
-
:From left; Sanple No. 1~7
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
 
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
 
-
|}
 
-
 
-
 
-
 
-
 
-
 
-
{|align="justify"
 
-
|[[Image:Chiba-0823-2.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>8~21</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>12</td>
 
-
</tr>
 
-
</table>
 
-
 
-
:From left;
 
-
:(Single Digestion) Sanple No. 8~14
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
 
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
 
-
 
-
:(Double Digestion) Sample No. 15~21
 
-
::*BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
 
-
::*BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
 
-
::*BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
 
-
::*BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
 
-
::*BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
 
-
::*BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
 
-
::*BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?
 
-
|}
 
-
 
-
 
-
 
-
 
-
:'''[[Team:Chiba/protocol/digestion|Digestion test]]'''
 
-
::BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
 
-
 
-
<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>1</td><td>2</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>3</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>EcoRⅠ</td>
 
-
<td>-</td><td>0.1</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>SpeⅠ</td>
 
-
<td>-</td><td>-</td><td>0.1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>EcoRⅠ Buffer</td>
 
-
<td>-</td><td>0.9</td><td>0.8</td>
 
-
</tr>
 
-
<tr>
 
-
<td>BSA</td>
 
-
<td>-</td><td>1</td><td>1</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>-</td><td>5</td><td>5</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>3</td><td>10</td><td>10</td>
 
-
</tr>
 
-
</table>
 
-
 
-
 
-
--->'''[[Team:Chiba/protocol/gelcheck|Gel Check]]'''
 
-
{|align="justify"
 
-
|[[Image:Chiba-0823-3.JPG]]
 
-
|
 
-
:<table width="315" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000">
 
-
<tr>
 
-
<td width="257">Sample No</td>
 
-
<td>1</td><td>2</td><td>3</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Sample DNA</td>
 
-
<td>3</td><td>10</td><td>10</td>
 
-
</tr>
 
-
<tr>
 
-
<td>Loading Dye</td>
 
-
<td>1</td><td>2</td><td>2</td>
 
-
</tr>
 
-
<tr>
 
-
<td>dH2O</td>
 
-
<td>2</td><td>-</td><td>-</td>
 
-
</tr>
 
-
<tr>
 
-
<td>TOTAL</td>
 
-
<td>6</td><td>12</td><td>12</td>
 
-
</tr>
 
-
</table>
 
-
:From left;
 
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
 
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
 
-
::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
 
-
|}
 
-
===[[Team:Chiba/jk/β/weak 2|>next week]]===
+
===[[Team:Chiba/jk/γ/weak 2|>next week]]===
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
{| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"

Revision as of 08:38, 25 September 2008

>出力班

Contents

Week 1

17,August 2008


PCR
BBa_E0430①
BBa_E0420②
BBa_J06702③
BBa_S01416④
BBa_I73003⑤
BBa_I73004⑥
BBa_I730026⑦
BBa_I763002⑧

>
Sample No ①②③④⑤⑥⑦⑧
DNA tamplate 11111111
FW primer 55555555
VR primer 55555555
dNTPmix 1010101010101010
thermo pol buffer 1010101010101010
DNA pol(VENT) 11111111
dH2O 6868686868686868
TOTAL 100100100100100100100100


20,August 2008


TRANSFORMATION
BBa_J52008(Luciferase Renilla)-->colony
BBa_I712019(Luciferase Fire fly)-->no colony
BBa_J332002(LacZ)-->colony
BBa_E2030(Venus YFP)-->not so good
YFP(P-GEX)-->colony
BBa_F2620-->colony
BBa_T9002-->colony


21,August 2008


TRANSFORMATION
BBa_J32007(Lux CDABE)-->no colony
BBa_B0034(RBS)-->colony


22,August 2008


MINI PREP
BBa_J52008(Luciferase)
BBa_F2620
BBa_T9002
BBa_J33202(LacZ)
PUC19
PGEX VENUS YFP(GST fusion)

TRANSFORMATION
PGEX VENUS YFP(GST fusion)


>next week

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