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Team:Warsaw/Calendar-Main/29 May 2008
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| + | Piotr: <br> | ||
| + | 1. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB. | ||
| + | 2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose. | ||
| - | + | 3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB. | |
| - | + | 4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG. | |
| - | + | 5. Wait for 5 hours. | |
| - | + | 6. OD measurement. | |
| - | + | 7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin). | |
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| - | 7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin) | + | |
Revision as of 13:32, 1 October 2008
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Piotr: 2. Add 50 μL E.coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose. 3. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB. 4. Add 50 μL E.coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1mM IPTG. 5. Wait for 5 hours. 6. OD measurement. 7. 1 ml (2x) from p.1, p.2, p.3, p.4 (LB + rifampicin).
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