Team:Warsaw/Calendar-Main/14 July 2008

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(New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Ligation of protein Z DNA to OmpA constructs'''<br> 1. Digestation of Geneart_Z, </p> <!-- do not remove this! --> {{WarNoteboo...)
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<p>'''Ligation of protein Z DNA to OmpA constructs'''<br>
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<p>'''Cloning of protein Z DNA to OmpA constructs'''<br>
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1. Digestation of Geneart_Z, </p>
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1. Digestation and dephosphorylatio (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. <br> </p>
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2. Gel eloctrophoresis and gel-out (4300 bp band). <br>
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3. Ligation of isolated DNA fragment and Z DNA fragment isolated on

Revision as of 15:15, 26 September 2008

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Cloning of protein Z DNA to OmpA constructs
1. Digestation and dephosphorylatio (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.

2. Gel eloctrophoresis and gel-out (4300 bp band).
3. Ligation of isolated DNA fragment and Z DNA fragment isolated on



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