Team:Warsaw/Calendar-Main/30 June 2008

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<p>'''Optimisation of primers from 19.06'''<br>
<p>'''Optimisation of primers from 19.06'''<br>
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1. PCR on pBD (jaki numerek?) plasmid with
+
1. PCR on pDRIVE-TapTag plasmid using
<html>
<html>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
</html>
</html>
  primers (annealing length 45s)<br>
  primers (annealing length 45s)<br>
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2. PCR on pUC19 plasmid with
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2. PCR on pUC19 plasmid using
<html>
<html>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">AlphaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">AlphaP_XB</a>
</html>
</html>
primers (annealing length 45s)<br>
primers (annealing length 45s)<br>
-
3. PCR on pUC19 plasmid with
+
3. PCR on pUC19 plasmid using
<html>
<html>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>

Revision as of 18:32, 1 October 2008

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Optimisation of primers from 19.06
1. PCR on pDRIVE-TapTag plasmid using OmpaL_N and OmpaP_link primers (annealing length 45s)
2. PCR on pUC19 plasmid using AlphaL_link and AlphaP_XB primers (annealing length 45s)
3. PCR on pUC19 plasmid using OmegaL_link and OmegaP_EPB primers (annealing length 30s)
Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees