Team:Warsaw/Calendar-Main/18 July 2008

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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
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2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate)inoculated to liquid LB with kanamycin. <br>
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Cloning of protein A DNA to OmpA constructs
1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_A_omega and pACYC177+OmpA_A_alpha. Primers used: AL+SacI and AP+NotI
2. Confirmed transformant colonies (found only on pACYC177+OmpA_A_alpha plate)inoculated to liquid LB with kanamycin.



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