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Team:Warsaw/Calendar-Main/22 May 2008
From 2008.igem.org
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| + | Michał K.: | ||
| + | 1. PCR - transcription fusion: AID + T7 RNA-polymerase</p> | ||
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| + | <p>Primers: </p> | ||
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| + | <p><html> | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and | ||
| + | <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></html></p> | ||
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| + | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
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| + | Annealing temperature : | ||
| + | Annealing time: 4 minutes <br> | ||
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| + | 35 cycles <br> | ||
Revision as of 21:50, 30 September 2008
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Piotr: 1. Design of primers for sequencing something we want from pMPM-T5 Primers: Michał K.: 1. PCR - transcription fusion: AID + T7 RNA-polymerase</p> Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature :
Annealing time: 4 minutes 35 cycles
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