Team:Warsaw/Calendar-Main/5 May 2008

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<p>Piotr: Setup of overnight culture <i>E. coli</i> TOP10 and Rosetta on LB broth for preparation of chemocompetent bacteria.</p>
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<p>Piotr: Setup of overnight culture <i>E. coli</i> TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria.</p>
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'''TOP10 (Invitrogen)'''<br>
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F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ- <br>
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Piotr: Setup of overnight culture E. coli TOP10 (LB broth) and Rosetta (LB broth + chloramphenicol) for preparation of chemocompetent bacteria.


TOP10 (Invitrogen)

F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 nupG recA1 araD139 Δ(ara-leu)7697 galE15 galK16 rpsL(StrR) endA1 λ-
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