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Team:Warsaw/Calendar-Main/8 July 2008
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| - | < | + | <h3>Preparation of constructs with OmpA protein fusions</h3> |
| - | + | <p> | |
| - | + | <ol> | |
| + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation from previous day </li> | ||
| + | <li> Transformants plating on LB + kanamycin</li></ol></p> | ||
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| - | < | + | <h3>Preparation of construct pKS with A protein</h3> |
| - | + | <p><ol> | |
| - | + | <li> Inactivation of digestion enzymes and CIAP</li> | |
| - | + | <li> Ligation of digested PCR product and pKS 2h at room temperature</li> | |
| - | + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with 7 µl of ligation mix</li> | |
| + | <li> Transformants plating on LB + ampicillin + X-gal + IPTG</li></ol></p> | ||
Revision as of 17:20, 1 October 2008
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Preparation of constructs with OmpA protein fusions
Preparation of construct pKS with A protein
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