Team:Warsaw/Calendar-Main/9 July 2008

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<h3>Preparation of constructs with OmpA protein fusions</h3>
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<li> Colony PCR on colonies from plates with transformations pACYC177+OmpA_omega and pACYC177+OmpA_alpha Primers used:
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<p>'''Preparation of constructs with OmpA protein fusions'''<br>
 
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1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_omega and pACYC177+OmpA_alpha Primers used:
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpaL_N</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpaP_link</a>
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2. Confirmed transformant colonies inoculated to liquid LB with kanamycin</p>
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<li> Confirmed transformant colonies inoculated to liquid LB with kanamycin
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</li></ol></p>
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<p>'''Preparation of construct pKS with A protein''' <br>
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<h3>Preparation of construct pKS with A protein</h3>
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1. Colony PCR on colonies from plates with transformations pKS-A <br>
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<li> Colony PCR on colonies from plates with transformations pKS-A <br>
Primers used:
Primers used:
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a>
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2. Confirmed transformant colonies inoculated to liquid LB with ampicillin</p>
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<li> Confirmed transformant colonies inoculated to liquid LB with ampicillin</li></ol></p>
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Revision as of 17:25, 1 October 2008

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Preparation of constructs with OmpA protein fusions

  1. Colony PCR on colonies from plates with transformations pACYC177+OmpA_omega and pACYC177+OmpA_alpha Primers used: OmpaL_N and OmpaP_link
  2. Confirmed transformant colonies inoculated to liquid LB with kanamycin

Preparation of construct pKS with A protein

  1. Colony PCR on colonies from plates with transformations pKS-A
    Primers used: AL+SacI and AP+NotI
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin

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