"
Team:Warsaw/Calendar-Main/12 May 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
|||
| Line 2: | Line 2: | ||
<html> | <html> | ||
<p>Michał K.:</p> | <p>Michał K.:</p> | ||
| - | <p>1. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI.</p> | + | <p>1. Restriction digest of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a> with HindIII and NcoI (1x Tango buffer).</p> |
<p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band.</p> | <p>2. DNA electrophoresis and <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation of DNA</a> encoding AID from proper band.</p> | ||
Revision as of 19:08, 28 September 2008
 |
|
||||||||
 |
|
||||||||
Michał K.: 1. Restriction digest of pTrc99A-AID with HindIII and NcoI (1x Tango buffer). 2. DNA electrophoresis and isolation of DNA encoding AID from proper band. 3. Restriction digest of pMPM-T5 with HindIII and NcoI, use of alkaline phosphatase (CIAP). 4. DNA electrophoresis and isolation of pMPM-T5 vector from proper band. 5. DNA ligation (pMPM-T5+AID vector)  </html>
|
|
