Team:University of Sheffield /Project
From 2008.igem.org
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!align="center"|[[Team:University_of_Sheffield |Home]] | !align="center"|[[Team:University_of_Sheffield |Home]] | ||
!align="center"|[[Team:University_of_Sheffield /Project|Our project]] | !align="center"|[[Team:University_of_Sheffield /Project|Our project]] | ||
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|<H2>Project Overview</H2><br> | |<H2>Project Overview</H2><br> | ||
<p>We want is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing, namely the system in '''''Vibrio cholerae''''', the bacterial species that causes epidemics of cholera. Quorum sensing systems use sensing proteins in the membrane (<em>[http://en.wikipedia.org/wiki/Histidine_kinase histidine-kinases] in this case</em>) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each quorum-sensing species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA.</p> | <p>We want is to engineer a biological machine that will sense microbial contamination of drinking water using quorum sensing, namely the system in '''''Vibrio cholerae''''', the bacterial species that causes epidemics of cholera. Quorum sensing systems use sensing proteins in the membrane (<em>[http://en.wikipedia.org/wiki/Histidine_kinase histidine-kinases] in this case</em>) to detect molecules excreted by the bacteria’s own species. After detection in the membrane, a phosphate signal is passed down pathway, causing a DNA regulatory effect. Each quorum-sensing species also has its own corresponding quorum-producing protein. In Vibrio cholerae the sensing protein is CqsS and the quorum-producing protein CqsA.</p> | ||
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|<H2>If everything goes well...</H2><br> | |<H2>If everything goes well...</H2><br> | ||
<p>We are hijacking a pathway already present in E.coli – the non-essential BarA, pathway which is partly responsible for 'last resort' carbon metabolism. This pathway starts with similar histidine-kinase (<em>KdpD</em>) to Vibrio cholerae, and makes a compatible candidate topologically. We're taking the sending part of the Vibrio cholerae histidine kinase (called CqsS) and attaching it to the phosphotransfer part of the BarA histidine kinase, forming a new, fusion kinase. This is the simple way of putting it, as fusion proteins are notoriously difficult to get to work in reality. However the similiar topology between BarA and CqsS helps us somewhat, and sessions with Prof Rice (a protein crystollogropher in the Molecular Biology and Biotechnology department here) have increased our chances of a succesfulm protein. | <p>We are hijacking a pathway already present in E.coli – the non-essential BarA, pathway which is partly responsible for 'last resort' carbon metabolism. This pathway starts with similar histidine-kinase (<em>KdpD</em>) to Vibrio cholerae, and makes a compatible candidate topologically. We're taking the sending part of the Vibrio cholerae histidine kinase (called CqsS) and attaching it to the phosphotransfer part of the BarA histidine kinase, forming a new, fusion kinase. This is the simple way of putting it, as fusion proteins are notoriously difficult to get to work in reality. However the similiar topology between BarA and CqsS helps us somewhat, and sessions with Prof Rice (a protein crystollogropher in the Molecular Biology and Biotechnology department here) have increased our chances of a succesfulm protein. | ||
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|<H2>History of Development</H2><br> | |<H2>History of Development</H2><br> | ||
*[[Team:University_of_Sheffield / Initial_BrSt |Early Brainstorming & Other Crazy Ideas]] | *[[Team:University_of_Sheffield / Initial_BrSt |Early Brainstorming & Other Crazy Ideas]] | ||
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|<H2>More Details</H2> | |<H2>More Details</H2> | ||
[[Team:University_of_Sheffield / Parts|Parts submitted to the Registry]] | [[Team:University_of_Sheffield / Parts|Parts submitted to the Registry]] |
Revision as of 21:43, 28 September 2008
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