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Team:Warsaw/Calendar-Main/23 July 2008
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MKrzyszton (Talk | contribs) (New page: {{WarNotebook}} <!-- do not edit above me! --> <p>'''Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha''' <br> 1. DNA fragment of omega_A isolated from gel on 12 July digested with...) |
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3. Digestation (SacI and NotI) and dephosphorylation (CIAP) of pACYC177+OmpA_alpha. <br> | 3. Digestation (SacI and NotI) and dephosphorylation (CIAP) of pACYC177+OmpA_alpha. <br> | ||
4. Gel electrophoresis and gel-out of 4300 bp band. <br> | 4. Gel electrophoresis and gel-out of 4300 bp band. <br> | ||
| - | 5. Ligation of DNA fragments from 2. and 4. <br> | + | 5. Ligation of DNA fragments from 2. and 4. (1 hr)<br> |
| + | 6. Transformation of E. coli TOP10 strain with ligation. <br> | ||
| + | 7. Transformants plating on LB + kanamycin. | ||
</p> | </p> | ||
Revision as of 15:29, 28 September 2008
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Cloning of omega_A DNA fragment to pACYC177+OmpA_alpha
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