Team:Warsaw/Calendar-Main/6 May 2008

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<p>Piotr:  
<p>Piotr:  
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Preparation of  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">electro-</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10<br>
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Preparation of  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrocompetent">electro-</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#chemocompetent">chemocompetent</a> bacteria <i>E. coli</i> TOP10.<br>
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Piotr: Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics<br>
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Piotr: Setup of overnight cultures (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a>, <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a>) on LB broth with proper antibiotics.<br>
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Michał Lower: <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta)<br>
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Michał Lower: <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#genomic_DNA_isolation"> Isolation of genomic DNA</a> (<i>E.coli</i> Rosetta).<br>
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Revision as of 18:16, 28 September 2008

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Piotr: Preparation of electro- and chemocompetent bacteria E. coli TOP10.
Piotr: Setup of overnight cultures (pTrc99A-AID, pMPM-T5, pZC320) on LB broth with proper antibiotics.
Michał Lower: Isolation of genomic DNA (E.coli Rosetta).