Team:Warsaw/Calendar-Main/15 May 2008

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<p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</p>
<p>1. <a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.</p>
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<p>2. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestation</a> of plasmids with HindIII and NcoI (1xTango buffer) - construct control.</p>
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<p>2. Restriction <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of plasmids with HindIII and NcoI (1xTango buffer) - construct control.</p>
<p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p>
<p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p>

Revision as of 15:33, 1 October 2008

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Michał K.

1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.

2. Restriction digestion of plasmids with HindIII and NcoI (1xTango buffer) - construct control.

3. Gel electrophoresis - choice of proper clones (all checked colonies).

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