Team:Warsaw/Calendar-Main/16 May 2008
From 2008.igem.org
(Difference between revisions)
MKrzyszton (Talk | contribs) |
MKrzyszton (Talk | contribs) |
||
Line 39: | Line 39: | ||
20 cycles<br> | 20 cycles<br> | ||
+ | <br> | ||
3. PCR - T7 RNA-polimerase for transcription fusion | 3. PCR - T7 RNA-polimerase for transcription fusion |
Revision as of 23:09, 28 September 2008
Michał K.: 1. PCR - AID for translation fusion Primers:
AIDlNrH
AIDpLinB
Annealing time: 60 sec 20 cycles
Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles 3. PCR - T7 RNA-polimerase for transcription fusion Primers: Template DNA: E. coli Rosetta genomic DNA Annealing temperature: 62 °C Annealing time: 4 minutes 20 cycles
|