Team:Warsaw/Calendar-Main/16 May 2008

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(Difference between revisions)
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Michał K.:
Michał K.:
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1. PCR - AID for translation fusion
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1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">PCR</a> - AID for translation fusion
Primers:
Primers:

Revision as of 14:30, 29 September 2008

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Michał K.:

1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">PCR</a> - AID for translation fusion

Primers:

AIDlNrH AIDpLinB
Template DNA: pTrc99A-AID Annealing temperature: 55 °C

Elongation time: 60 sec

20 cycles


2. PCR - T7 RNA-polimerase for translation fusion

Primers:

T7lLinkB T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Elongation time: 4 minutes

20 cycles

3. PCR - T7 RNA-polimerase for transcription fusion

Primers:

T7lRBSHi T7pXbSal

Template DNA: E. coli Rosetta genomic DNA

Annealing temperature: 62 °C

Elongation time: 4 minutes

20 cycles