Team:Warsaw/Calendar-Main/19 May 2008

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<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p>
<p>1. Gel electrophoresis (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/16_May_2008">16 May</a> PCR's) and DNA <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">isolation</a> from proper bands.</p>
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<p>2. PCR - translation fusion: AID + T7 RNA-polymerase</p>
 
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<p>Primers: </p>
 
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<p><html>
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and
 
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a></p>
 
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</html>
 
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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion
 
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Annealing temperature: 55 °C
 
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Annealing time: 4 minutes
 
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20 cycles
 
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3. Gel electrophoresis and DNA isolation from proper band (translation fusion: AID + T7 RNA-polymerase)
 

Revision as of 13:18, 30 September 2008

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Michał K.:

1. Gel electrophoresis (16 May PCR's) and DNA isolation from proper bands.

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