Team:Warsaw/Calendar-Main/14 May 2008

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<p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br>
<p>1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).<br>
2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62&deg;C to 82&deg;C). <br>
2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62&deg;C to 82&deg;C). <br>
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Primers:
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<html>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a>
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<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDpLinB">AIDpLinB</a>
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<br>
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Template DNA: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99A-AID</a>
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</html> <br>
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Elongation time: 60 sec <br>
Elongation time: 60 sec <br>

Revision as of 22:04, 29 September 2008

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Michał K.:

1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C).
Primers: AIDlNrH AIDpLinB
Template DNA: pTrc99A-AID
Elongation time: 60 sec
20 cycles

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