Team:Warsaw/Calendar-Main/14 May 2008

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Elongation time: 60 sec <br>
Elongation time: 60 sec <br>
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20 cycles <br></p>
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3. Optimalization of conditions for PCR - T7 RNA polymerase for translational and transcriptional fusion. <br></p>
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Revision as of 22:07, 29 September 2008

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Michał K.:

1.Setup of 9 separate cultures from 9 colonies of transformants pMPMT5+AID (liquid LB+tetracycline).
2. Optimalization of conditions for PCR to obtain AID DNA fragment - annealing temperature gradient (from 62°C to 82°C).
Primers: AIDlNrH AIDpLinB
Template DNA: pTrc99A-AID
Elongation time: 60 sec
20 cycles
3. Optimalization of conditions for PCR - T7 RNA polymerase for translational and transcriptional fusion.

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