Team:Paris/Modeling/f5

From 2008.igem.org

(Difference between revisions)
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[[Image:f5DCA.png|thumb]]
[[Image:f5DCA.png|thumb]]
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<br><br>
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We have [FlhDC] = {coef<sub>flhDC</sub>}''expr(pTet)'' = {coef<sub>flhDC</sub>} &#131;1([aTc]<sub>i</sub>)
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 +
and [FliA] = {coef<sub>FliA</sub>}''expr(pBad)'' = {coef<sub>FliA</sub>} &#131;2([arab]<sub>i</sub>)
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 +
So, at steady-states,
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[[Image:F4.jpg|center]]
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{|border="1" style="text-align: center"
{|border="1" style="text-align: center"
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|unit
|unit
|value
|value
 +
|comments
|-
|-
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|[expr(pFliL)]
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|[expr(pFlhDC)]
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|expression rate of <br> pFliL '''with RBS E0032'''
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|expression rate of <br> pFlhDC '''with RBS E0032'''
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|nM.s<sup>-1</sup>
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|nM.min<sup>-1</sup>
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|see [[Team:Paris/Modeling/Programs|"findparam"]] <br> need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
+
|
 +
|need for 20 mesures with well choosen values of [aTc]<sub>i</sub> <br> and for 20 mesures with well choosen values of [arab]<sub>i</sub> <br> and 5x5 measures for the relation below?
|-
|-
|γ<sub>GFP</sub>
|γ<sub>GFP</sub>
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|min<sup>-1</sup>
|min<sup>-1</sup>
|0.0198
|0.0198
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|
|-
|-
|[GFP]
|[GFP]
|GFP concentration at steady-state
|GFP concentration at steady-state
|nM
|nM
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|
 +
|need for 20 + 20 measures <br> and 5x5 measures for the relation below?
|-
|-
|(''fluorescence'')
|(''fluorescence'')
|value of the observed fluorescence
|value of the observed fluorescence
|au
|au
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|need for 20 + 20 measures <br> and 5x5 measures for the ''SUM''?
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|
 +
|need for 20 + 20 measures <br> and 5x5 measures for the relation below?
|-
|-
|''conversion''
|''conversion''
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|nM.au<sup>-1</sup>
|nM.au<sup>-1</sup>
|(1/79.429)
|(1/79.429)
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|
|}
|}
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|unit
|unit
|value
|value
 +
|comments
|-
|-
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|β<sub>53</sub>
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|β<sub>13</sub>
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|production rate of FlhDC-pFliL '''with RBS E0032''' <br> β<sub>53</sub>
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|production rate of FliA-pFlhDC '''with RBS E0032''' <br> β<sub>13</sub>
|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
 +
|
|
|
|-
|-
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|(K<sub>47</sub>/{coef<sub>flhDC</sub>})
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|(K<sub>12</sub>/{coef<sub>fliA</sub>})
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|activation constant of FlhDC-pFliL <br> K<sub>47</sub>
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|activation constant of FliA-pFlhDC <br> K<sub>12</sub>
|nM
|nM
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|
|
|
|-
|-
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|n<sub>47</sub>
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|n<sub>12</sub>
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|complexation order of FlhDC-pFliL <br> n<sub>47</sub>
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|complexation order of FliA-pFlhDC <br> n<sub>12</sub>
|no dimension
|no dimension
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|
|
|
|-
|-
-
|-
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|β<sub>2</sub>
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|β<sub>54</sub>
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|production rate of OmpR-pFlhDC '''with RBS E0032''' <br> β<sub>2</sub>
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|production rate of FliA-pFliL '''with RBS E0032''' <br> β<sub>54</sub>
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|nM.min<sup>-1</sup>
|nM.min<sup>-1</sup>
 +
|
|
|
|-
|-
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|(K<sub>48</sub>/{coef<sub>fliA</sub>})
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|(K<sub>22</sub>/{coef<sub>omp</sub>})
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|activation constant of FliA-pFliL <br> K<sub>48</sub>
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|activation constant of OmpR-pFlhDC <br> K<sub>22</sub>
|nM
|nM
 +
|
|
|
|-
|-
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|n<sub>48</sub>
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|n<sub>22</sub>
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|complexation order of FliA-pFliL <br> n<sub>48</sub>
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|complexation order of OmpR-pFlhDC <br> n<sub>22</sub>
|no dimension
|no dimension
 +
|
|
|
|}
|}

Revision as of 18:33, 16 October 2008

F5DCA.png

We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)

and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)

So, at steady-states,

F4.jpg


param signification unit value comments
[expr(pFlhDC)] expression rate of
pFlhDC with RBS E0032 		nM.min-1 need for 20 mesures with well choosen values of [aTc]i
and for 20 mesures with well choosen values of [arab]i
and 5x5 measures for the relation below?
γGFP dilution-degradation rate
of GFP(mut3b) 		min-1 0.0198
[GFP] GFP concentration at steady-state nM need for 20 + 20 measures
and 5x5 measures for the relation below?
(fluorescence) value of the observed fluorescence au need for 20 + 20 measures
and 5x5 measures for the relation below?
conversion conversion ratio between
fluorescence and concentration 		nM.au-1 (1/79.429)



param signification
corresponding parameters in the equations 		unit value comments
β13 production rate of FliA-pFlhDC with RBS E0032
β13 		nM.min-1
(K12/{coeffliA}) activation constant of FliA-pFlhDC
K12 		nM
n12 complexation order of FliA-pFlhDC
n12 		no dimension
β2 production rate of OmpR-pFlhDC with RBS E0032
β2 		nM.min-1
(K22/{coefomp}) activation constant of OmpR-pFlhDC
K22 		nM
n22 complexation order of OmpR-pFlhDC
n22 		no dimension



Then, if we have time, we want to verify the expected relation

SumpFliL.jpg
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