Team:Chiba/jk/γ/week 3

From 2008.igem.org

(Difference between revisions)

Revision as of 06:12, 24 October 2008

WEEK 3

31,August,2008

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②

Sample No.	①	②
DNA	10	10
SpeⅠ	1	0
PstⅠ	1	1
XbaⅠ	0	1
BSA(x100)	0.5	0.5
Buffer2	5	0
Buffer3	0	5
dH₂O	28.5	28.5
TOTAL	50	50

-->37℃ 3hour

-->①20μl refine

-->②20μl refine

1,September,2008

Ligation

vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
negative control:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]②

</tr>

Sample No.	①	②
vector	2	2
insert	3	0
Ligase Buffer	2	2
Ligase	1	1
dH₂O	3	5
TOTAL	10	10

-->R/T 2hour

TRANSFORMATION
BBa_R0079(Las promoter)
BBa_R0071(RhlR promoter)
BBa_R0077(cinR promoter+RBS)
BBa_R0078(cnR promoter)
BBa_R0062(LUX promoter)

2,September,2008

TIME RESPONCE (Solid)

COLONY PCR
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]+[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]

</tr>

Sample No.	①
culture	1
Fwd primer	1.5
Rev primer	1.5
Thermo pol Buffer	3
dNTP mix	3
Taq DNA pol (NEB)	0.2 (1 unit)
dH₂O	19.8
TOTAL	30

MINI Prep
BBa_R0079
BBa_R0071
BBa_R0077
BBa_R0078
BBa_R0062

3,September,2008

Digestion test
BBa_R0079①
BBa_R0071②
BBa_R0077③
BBa_R0078④
BBa_R0062⑤

</tr>

Sample No.	①~⑤
DNA tamplate	5
Buffer3	1
dH₂O	4
EcoRⅠ	0.1
TOTAL	10

->37℃30min
->Gel cheack

Sample No.	①~⑤
DNA(digestion sample)	10
loading Dye	2
TOTAL	12

4,Septempber,2008

TANSFORMATION
LIgation product
rLUciferase([http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] ) x3
Venus YFP([http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]) x3

5,September,2008

TIME RESPONCE(liquid)
Digestion test
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2

DNA(digestion sample)	2
PstⅠ	0.1
BSA	0.8
Buffer3	1
dH₂O	7
TOTAL	10

--->37℃ 30in
Gel Cheack
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2①
[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2②
[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](PCR Product)③
BBa_E2050④

Sample No.	①~②	③,④
DNA(digestion sample)	10	1
loading Dye	2	1
TOTAL	12	6

Luciferase cheack->no light

>Last Week
>Next Week

ホーム	メンバー紹介	プロジェクト紹介	Parts Submitted to the Registry	モデリング	ノート

@@ Line 9: / Line 9: @@
 == 31,August,2008 ==
-<br>digestion
+[[Team:Chiba/protocol/digestion|Digestion]]
-<br>BBa_R0010①
-<br>PCR product(BBa_J52008)②
+*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]①
+*PCR product([http://partsregistry.org/Part:BBa_J52008 BBa_J52008])②
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td>
 </tr>
@@ Line 46: / Line 48: @@
 </tr>
 <tr>
-<td>dH20</td>
+<td>dH<sub>2</sub>O</td>
 <td>28.5</td><td>28.5</td>
 </tr>
@@ Line 54: / Line 56: @@
 </tr>
 </table>
-<br>->37℃ 3hour
-<br>->①20μl refine
+-->37℃ 3hour
-<br>->②20μl refine
+-->①20μl refine
+-->②20μl refine
 == 1,September,2008 ==
-<br>Ligation
+[[Team:Chiba/protocol/ligation/ligation|Ligation]]
-<br>vector:BBa_R0010 insert:BBa_J52008①
+*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]①
-<br>negative control:BBa_R0010②
+*negative control:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]②
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td><td>②</td>
 </tr>
@@ Line 86: / Line 91: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>3</td><td>5</td>
 </tr>
@@ Line 93: / Line 98: @@
 </tr>
 </table>
-<br>->R/T 2hour
+-->R/T 2hour
 <br>TRANSFORMATION
@@ Line 110: / Line 116: @@
 <br>COLONY PCR
-<br>BBa_R0010+BBa_J52008
+<br>[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]+[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①</td>
 </tr>
@@ Line 140: / Line 146: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>19.8</td>
 </tr>
@@ Line 156: / Line 162: @@
 == 3,September,2008 ==
-<br>Digestion test
+[[Team:Chiba/protocol/digestion|Digestion]] test
 <br>BBa_R0079①
 <br>BBa_R0071②
@@ Line 164: / Line 170: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~⑤</td>
 </tr>
@@ Line 176: / Line 182: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>4</td>
 </tr>
@@ Line 191: / Line 197: @@
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~⑤</td>
 </tr>
@@ Line 213: / Line 219: @@
 <br>TANSFORMATION
 <br>LIgation product
-<br>rLUciferase(BBa_J52008+BBa_F2620 )   x3
+<br>rLUciferase([http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] )   x3
-<br>Venus YFP(BBa_E2030+BBa_F2620)    x3
+<br>Venus YFP([http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620])    x3
 == 5,September,2008 ==
 <br>[[Team:Chiba/jk/γ/Trl|TIME RESPONCE(liquid)]]
-<br>Digestion test
+<br>[[Team:Chiba/protocol/digestion|Digestion]] test
-<br>BBa_J52008+BBa_F2620 x2
+<br>[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2
-<br>BBa_E2030+BBa_F2620  x2
+<br>[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]  x2
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
@@ Line 241: / Line 247: @@
 </tr>
 <tr>
-<td>dH2O</td>
+<td>dH<sub>2</sub>O</td>
 <td>7</td>
 </tr>
@@ Line 252: / Line 258: @@
 <br>--->37℃ 30in
 <br>Gel Cheack
-<br>BBa_J52008+BBa_F2620 x2①
+<br>[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620] x2①
-<br>BBa_E2030+BBa_F2620  x2②
+<br>[http://partsregistry.org/Part:BBa_E2030 BBa_E2030]+[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]  x2②
-<br>BBa_J52008(PCR Product)③
+<br>[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](PCR Product)③
 <br>BBa_E2050④
 <table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 <tr>
-<td width="257">Sample No</td>
+<td width="257">Sample No.</td>
 <td>①~②</td><td>③,④</td>
 </tr>

Team:Chiba/jk/γ/week 3

From 2008.igem.org

Revision as of 06:12, 24 October 2008

Contents

WEEK 3

31,August,2008

1,September,2008

2,September,2008

3,September,2008

4,Septempber,2008

5,September,2008