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Team:Warsaw/Calendar-Main/22 May 2008
From 2008.igem.org
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Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion <br> | ||
| - | Annealing temperature : | + | Annealing temperature : 60&dgr;C <br> |
Annealing time: 4 minutes <br> | Annealing time: 4 minutes <br> | ||
Revision as of 21:58, 30 September 2008
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Piotr: 1. Design of primers for sequencing something we want from pMPM-T5 Primers: Michał K.: 1. PCR - transcription fusion: AID + T7 RNA-polymerase</p> Primers:
Template DNA: AID for translation fusion and T7 RNA-polymerase for translation fusion Annealing temperature : 60&dgr;C 35 cycles
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