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Team:Warsaw/Calendar-Main/23 May 2008
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<p>Michał K.:</p> | <p>Michał K.:</p> | ||
| - | <p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation> of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI</p> | + | <p>1. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI</p> |
| - | <p>2. | + | <p>2. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI</p> |
<p>3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested products from p.1 and p.2</p> | <p>3. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of digested products from p.1 and p.2</p> | ||
| - | <p>4. | + | <p>4. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of pMPM-T5+AID with HindIII and SalI</p> |
| - | <p>5. | + | <p>5. <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">Digestation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPM-T5</a> with NcoI and XbaI</p> |
<p>6. Gel-electrophresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands.</p> | <p>6. Gel-electrophresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands.</p> | ||
Revision as of 22:42, 30 September 2008
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Michał K.: 1. Digestation of PCR product for transcription fusion (T7 RNA-polimerase) with HindIII and SalI 2. Digestation of PCR product for translation fusion (AID+T7 RNA-polimerase) with NcoI and XbaI 3. Clean-up of digested products from p.1 and p.2 4. Digestation of pMPM-T5+AID with HindIII and SalI 5. Digestation of pMPM-T5 with NcoI and XbaI 6. Gel-electrophresis and gel-out of proper bands. 7. Ligation of purified products from p.1 and p.4 8. Electroporation of E.coli TOP10 with ligation product 9. Ligation of digested products from p.4 and p.5 10. Electroporation of E.coli TOP10 with ligation product 11. Transformants plating on LB + tetracycline http://openwetware.org/images/thumb/6/61/UW_A%2BT.jpg/350px-UW_A%2BT.jpg http://openwetware.org/images/thumb/c/c7/UW_AT.jpg/350px-UW_AT.jpg
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