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Team:HKUSTers
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|[[Image:logo_ust(s).jpg|right|frame|Our logo:The ''Bauhinia purpurea x variegata'' at the top-left corner is the floral emblem of Hong Kong; the red sundial on the left represents HKUST, as there is a large sundial in HKUST as a landmark; the Chinese brush style English letters reflect the cultural diversity of Hong Kong as a place where east meets west.]] | |[[Image:logo_ust(s).jpg|right|frame|Our logo:The ''Bauhinia purpurea x variegata'' at the top-left corner is the floral emblem of Hong Kong; the red sundial on the left represents HKUST, as there is a large sundial in HKUST as a landmark; the Chinese brush style English letters reflect the cultural diversity of Hong Kong as a place where east meets west.]] | ||
| - | '''Abstract''' | + | [['''Abstract''']] |
| - | '''Does God play dice with the cell?''' | + | '''''Does God play dice with the cell?''''' |
| - | Stochastic fluctuation in a cellular context and the lambda-phage bifurcation have been extensively studied. However, from a bottom-up synthetic aspect, we aim to exploit the cellular "noise" to build an E. coli version of a computational device, the "Random Number Generator". One random binary digit can be generated by capturing an initial Polymerase binding event with a pair of mutually exclusive promoters. Reciprocal inhibition using two repressors shall achieve | + | Stochastic fluctuation in a cellular context and the lambda-phage |
| - | + | bifurcation have been extensively studied. However, from a bottom-up | |
| - | Balancing the two sets of affinity and kinetic parameters and maintaining a single copy of this synthetic device integrated into the bacterial chromosome shall improve performance. If successful, coupled with other reporters we envision multiple extensions of this "Randomizer", including a Memorizer that utilizes a hierarchy of XOR- | + | synthetic aspect, we aim to exploit the cellular "noise" to build an |
| + | E. coli version of a computational device, the "Random Number | ||
| + | Generator". One random binary digit can be generated by capturing an | ||
| + | initial Polymerase binding event with a pair of mutually exclusive | ||
| + | promoters. Reciprocal inhibition using two repressors shall achieve | ||
| + | unilateral expression of the "switch", with fluorescence reporters | ||
| + | indicating the probability of each alternative occurrence. | ||
| + | Balancing the two sets of affinity and kinetic parameters and | ||
| + | maintaining a single copy of this synthetic device integrated into the | ||
| + | bacterial chromosome shall improve performance. If successful, coupled | ||
| + | with other reporters we envision multiple extensions of this | ||
| + | "Randomizer", including a Memorizer that utilizes a hierarchy of | ||
| + | XOR-calculations to "store" a multi-digit random number, and | ||
| + | intriguing pattern generation involving chemical gradients and random | ||
| + | "population behavior". | ||
<!--- The Mission, Experiments ---> | <!--- The Mission, Experiments ---> | ||
[[Image:top_03.gif]] We would truly like to thank '''Lee Hysan Foundations''' for their generous sponsorships! | [[Image:top_03.gif]] We would truly like to thank '''Lee Hysan Foundations''' for their generous sponsorships! | ||
Revision as of 03:07, 1 October 2008
Probably the most beautiful harbour in the world: Victoria Harbour, taken at the Peak, Hong Kong.
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| .jpg) Our logo:The Bauhinia purpurea x variegata at the top-left corner is the floral emblem of Hong Kong; the red sundial on the left represents HKUST, as there is a large sundial in HKUST as a landmark; the Chinese brush style English letters reflect the cultural diversity of Hong Kong as a place where east meets west. [[Abstract]] Does God play dice with the cell? Stochastic fluctuation in a cellular context and the lambda-phage bifurcation have been extensively studied. However, from a bottom-up synthetic aspect, we aim to exploit the cellular "noise" to build an E. coli version of a computational device, the "Random Number Generator". One random binary digit can be generated by capturing an initial Polymerase binding event with a pair of mutually exclusive promoters. Reciprocal inhibition using two repressors shall achieve unilateral expression of the "switch", with fluorescence reporters indicating the probability of each alternative occurrence. Balancing the two sets of affinity and kinetic parameters and maintaining a single copy of this synthetic device integrated into the bacterial chromosome shall improve performance. If successful, coupled with other reporters we envision multiple extensions of this "Randomizer", including a Memorizer that utilizes a hierarchy of XOR-calculations to "store" a multi-digit random number, and intriguing pattern generation involving chemical gradients and random "population behavior".
 We would truly like to thank Lee Hysan Foundations for their generous sponsorships!
|
