Team:Warsaw/Calendar-Main/25 June 2008

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<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br>
<p>'''Preparation of pMPMT5-AID+AIDT7 construct''' <br>
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1. Digestion of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP <br>
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1. Digest of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP <br>
2. Clean-up of digested PCR product. <br>
2. Clean-up of digested PCR product. <br>
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (4800 bp)<br>
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (4800 bp)<br>

Revision as of 17:55, 1 October 2008

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Preparation of pMPMT5-AID+AIDT7 construct
1. Digest of isolated PCR product (from previous day) and pMPMT5-AID with HindIII and XbaI, dephosphorylation of pMPMT5-AID with CIAP
2. Clean-up of digested PCR product.
3. Gel electrophoresis of digested pMPMT5-AID and gel-out of proper band (4800 bp)
4. Overnight ligation of purified DNA fragments



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