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Team:Warsaw/Calendar-Main/15 May 2008
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<p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p> | <p>3. Gel electrophoresis - choice of proper clones (all checked colonies).</p> | ||
| + | <p>MichałL, Ewa, Marcin.</p> | ||
| + | <p>Overnight inoculation and induction:</p> | ||
| + | <p>Top10 + 0,5 mM IPTG</p> | ||
| + | <p>pMPMAID + Glc + 0,01% L-Ara</p> | ||
| + | <p>pTRCAID + Glc + 0,5 mM IPTG</p> | ||
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Revision as of 15:43, 1 October 2008
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Michał K. 1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day. 2. Restriction digestion of plasmids with HindIII and NcoI (1xTango buffer) - construct control. 3. Gel electrophoresis - choice of proper clones (all checked colonies). MichałL, Ewa, Marcin. Overnight inoculation and induction: Top10 + 0,5 mM IPTG pMPMAID + Glc + 0,01% L-Ara pTRCAID + Glc + 0,5 mM IPTG |
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