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Team:Warsaw/Calendar-Main/27 May 2008
From 2008.igem.org
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<p>2. <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion.</p> | <p>2. <a href:"https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of hypothetical pMPM-T5 with translation fusion.</p> | ||
| - | <p>3. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> | + | <p>3. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer).</p> |
| - | <p>4. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> | + | <p>4. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with BamHI (BamHI buffer).</p> |
| - | <p>5. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> | + | <p>5. Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer).</p> |
<p>6. Choice of proper clones and preparation for sequencing.</p> | <p>6. Choice of proper clones and preparation for sequencing.</p> | ||
Revision as of 17:52, 1 October 2008
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Michał K.: 1. Isolation of hypothetical pMPM-T5 with transcription fusion. 2. Isolation of hypothetical pMPM-T5 with translation fusion. 3. Control digest of pMPM-T5 with transcription fusion with EcoRI and XhoI (2x Tango buffer). 4. Control digest of pMPM-T5 with translation fusion with BamHI (BamHI buffer). 5. Control digest of pMPM-T5 with translation fusion with EcoRI and XbaI (2x Tango buffer). 6. Choice of proper clones and preparation for sequencing. Here we found the clone with translational fusion on pMPM. These are different isolations in each lane. We have been working with the AT6 ever since.
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