Team:Warsaw/Calendar-Main/5 July 2008

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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)</li>  
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)</li>  
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digestion</a> of plasmids with NotI and BamHI</li>
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<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with NotI and BamHI</li>
<li> Gel electrophoresis </li>
<li> Gel electrophoresis </li>
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</ol></p>

Revision as of 17:57, 1 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega)
  2. Control digest of plasmids with NotI and BamHI
  3. Gel electrophoresis


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