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Team:Warsaw/Calendar-Main/14 July 2008
From 2008.igem.org
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<h3>Cloning of protein Z DNA to OmpA constructs</h3> | <h3>Cloning of protein Z DNA to OmpA constructs</h3> | ||
<p><ol> | <p><ol> | ||
| - | <li> Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI. </li> | + | <li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing">dephosphorylation (with CIAP)</a> of pACYC177+OmpA_alpha with SacI and NotI. </li> |
| - | <li> Gel eloctrophoresis and gel-out (4300 bp band). </li> | + | <li> Gel eloctrophoresis and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> (4300 bp band). </li> |
| - | <li> Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July. </li> | + | <li> |
| - | <li> Transformation of E. coli TOP10 strain with ligation. </li> | + | <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment and Z DNA fragment isolated on 10 July. </li> |
| + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation. </li> | ||
<li> Transformants plating on LB + kanamycin. </li> | <li> Transformants plating on LB + kanamycin. </li> | ||
Revision as of 18:13, 1 October 2008
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Cloning of protein Z DNA to OmpA constructs
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