Team:Warsaw/Calendar-Main/22 July 2008

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(Difference between revisions)
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2. Control digest of isolated plasmids with BamHI and SacI . </p>
2. Control digest of isolated plasmids with BamHI and SacI . </p>
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<p>Paweł: ligation transformed into TOP10 strain, plated on kanamycin plate</p>
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<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
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Paweł</h3>
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<p><ol><li>Results of ligation: 6 colonies grown.</li>
 +
<li>Each colony cultured overnight in LB + ampicillin</li></ol></p>

Revision as of 16:52, 2 October 2008

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Cloning of protein A DNA to OmpA constructs
1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
2. Control digest of isolated plasmids with BamHI and SacI .

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin

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