Team:Warsaw/Calendar-Main/3 July 2008

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<li> <https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digestion </li>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_purification_after_enzymatic_reaction">Clean-up</a> of overnight digest </li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b </li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_alfa and pET15b </li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b</li>
<li> <A HREF="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</A> of OmpA_omega and pET15b</li>

Revision as of 18:25, 1 October 2008

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Preparation of constructs with OmpA protein fusions
Michał K.

  1. Clean-up of overnight digest
  2. Ligation of OmpA_alfa and pET15b
  3. Ligation of OmpA_omega and pET15b
  4. Transformation of E. coli TOP10 strain with ligation products
  5. Transformants plating on LB + ampicillin

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