Team:Warsaw/Calendar-Main/10 June 2008

From 2008.igem.org

(Difference between revisions)
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
-
<li>Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.</li>
+
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.</li>
-
<li>Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.</li>
+
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + T7 RNA-polymerase with EcoRI.</li>
-
<li>Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.</li>
+
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.</li>
-
<li>DNA gel electrophoresis of digestion products.</li>
+
<li>DNA gel electrophoresis of digest products.</li>
<li>Picking out proper colonies of transformants.</li>
<li>Picking out proper colonies of transformants.</li>

Revision as of 18:43, 1 October 2008

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Michał L., Ewa, Marcin

  • Plasmid isolation from overnight culture of our transformants.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
  • Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
  • Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
  • DNA gel electrophoresis of digest products.
  • Picking out proper colonies of transformants.