Team:Warsaw/Calendar-Main/25 July 2008

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2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).   
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).   
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<p>Gel-out of Z (~200 bp band). Ligation of degested pET15b+OmpA+omega with Z. Ligations transformed into TOP10 strain and plated on LB+ampicillin. </p>
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Revision as of 17:02, 2 October 2008

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1. Isolation of plasmids from cultures inocluated on previous day.
2. Control digest of isolated plasmids with BamHI and SacI (we confirmed 4 colonies but A in our constructs turned out to be trunctated and contain only one from two highly similar domains).



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