Team:Warsaw/Calendar-Main/10 June 2008

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<html><h3>Michał L., Ewa, Marcin</h3>
<html><h3>Michał L., Ewa, Marcin</h3>
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<ul><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.</li>
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
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<li>Picking out proper colonies of transformants.</li>
<li>Picking out proper colonies of transformants.</li>
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Revision as of 23:37, 4 October 2008

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Michał L., Ewa, Marcin

  1. Plasmid isolation from overnight culture of our transformants.
  2. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.
  3. Control digest of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.
  4. Control digest of pMPM-T5 + T7 RNA-polymerase with EcoRI.
  5. Control digest of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.
  6. DNA gel electrophoresis of digest products.
  7. Picking out proper colonies of transformants.

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