Team:Warsaw/Calendar-Main/16 June 2008

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<h3>Michał L., Ewa, Marcin</h3>
<h3>Michał L., Ewa, Marcin</h3>
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<p><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">Colony PCR</a><br>
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<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a><br>
Template: DNA isolated from white colonies<br>
Template: DNA isolated from white colonies<br>
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Primers: PZCseqL and PZCseqR<br>
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Primers: <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqR">pZCseqR</a><br>
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Annealing time:
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Annealing time:<br>
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Number of cycles:</p>
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Number of cycles:</li>
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<p>DNA gel electrophoresis of PCR products.</p>
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<li>DNA gel electrophoresis of PCR products.</li>
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<p>Gel-out of proper products (~1200 bp).</p>
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<li>Gel-out of proper products (~1200 bp).</li>
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<p>Sequencing of proper fragments using primer PZCseqL.</p>
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<li>Sequencing of proper fragments using primer <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#pZCseqL">pZCseqL</a>.</li>
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Revision as of 23:54, 4 October 2008

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Michał L., Ewa, Marcin

  1. Colony PCR
    Template: DNA isolated from white colonies
    Primers: pZCseqL and pZCseqR
    Annealing time:
    Number of cycles:
  2. DNA gel electrophoresis of PCR products.
  3. Gel-out of proper products (~1200 bp).
  4. Sequencing of proper fragments using primer pZCseqL.

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