Team:Warsaw/Calendar-Main/2 June 2008

From 2008.igem.org

(Difference between revisions)
Line 17: Line 17:
Primers: <br>
Primers: <br>
-
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
+
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AIDlNrH">AIDlNrH</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a><br>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)<br>

Revision as of 18:56, 1 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Piotr:

  1. Transformation of E. coli TOP10 with pZC320.
  2. Plating transformants on LB + ampicillin(30μg/mL) + X-gal + IPTG.

PCR for AID transcription fusion with translation fusion AID+T7 RNA-polymerase
Michał K.:

Primers:
AIDlNrH and T7pXbSal
Template DNA: translation fusion AID+T7 RNA-polymerase (pMPM-T5)
Elongation temperature: 55°C
Time: 4 minutes
Number of cycles: 20


Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/2_June_2008"