Team:Warsaw/Calendar-Main/15 May 2008

From 2008.igem.org

(Difference between revisions)
Line 12: Line 12:
<li> Gel electrophoresis - choice of proper clones (all checked colonies). </li>
<li> Gel electrophoresis - choice of proper clones (all checked colonies). </li>
 +
<li> Optimization of conditions for <html><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62&deg;C to 82&deg;C). <br>
 +
Primers:
 +
 +
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7lLinkB">T7lLinkB</a>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#T7pXbSal">T7pXbSal</a>
 +
<br>
 +
 +
 +
Template DNA: <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> genomic DNA<br>
 +
 +
Elongation time: 4 minutes
 +
<br>
 +
 +
20 cycles<br>
</ol></p>
</ol></p>
</html>
</html>
<!-- do not remove this! -->
<!-- do not remove this! -->
{{WarNotebookEnd}}
{{WarNotebookEnd}}

Revision as of 20:53, 4 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Michał K.:

  1. Isolation of hypothetical pMPM-T5+AID plasmids from cultures inoculated on previous day.
  2. Restriction digest of plasmids with HindIII and NcoI (1xTango buffer) - construct control.
  3. Gel electrophoresis - choice of proper clones (all checked colonies).
  4. Optimization of conditions for PCR - T7 RNA polymerase for translation fusion; annealing temperature gradient (from 62°C to 82°C).
    Primers: T7lLinkB T7pXbSal
    Template DNA: E. coli Rosetta genomic DNA
    Elongation time: 4 minutes
    20 cycles

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008"