Cloning of protein Z DNA to OmpA constructs
Michał K.

1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin

Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA
Antoni

1. Isolation of plasmids from bacterial cultures inoculated on previous day (pKS+A and pGeneart+Z).
2. Digest of pKS+A and pGeneart+Z with SacI and NotI, pGeneart dephosphorylation with CIAP.
3. Gel electrophoresis of digested plasmids and gel-out of proper bands
4. Overnight ligation of pGeneart and A