Team:Warsaw/Calendar-Main/14 July 2008

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<h3>Cloning of protein A DNA to pET15b+OmpA-alfa plasmid in place of OmpA<br>Antoni</h3>
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<li>Inoculated to liquid LB + ampicilin pGeneart+Z and pKS+A</li>
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Revision as of 23:37, 2 October 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
  2. Gel eloctrophoresis and gel-out (4300 bp band).
  3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
  4. Transformation of E. coli TOP10 strain with ligation.
  5. Transformants plating on LB + kanamycin.



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