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Team:Warsaw/Calendar-Main/8 July 2008
From 2008.igem.org
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| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm# | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation from previous day. </li> |
<li> Transformants plating on LB + kanamycin.</li></ol></p> | <li> Transformants plating on LB + kanamycin.</li></ol></p> | ||
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<li> Inactivation of digestion enzymes and CIAP.</li> | <li> Inactivation of digestion enzymes and CIAP.</li> | ||
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested PCR product and pKS 2h at room temperature.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested PCR product and pKS 2h at room temperature.</li> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm# | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with 7 µl of ligation mix.</li> |
<li> Transformants plating on LB + ampicillin + X-gal + IPTG.</li></ol></p> | <li> Transformants plating on LB + ampicillin + X-gal + IPTG.</li></ol></p> | ||
Revision as of 21:44, 5 October 2008
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Preparation of constructs with OmpA protein fusions
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