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Team:Warsaw/Calendar-Main/29 July 2008
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<p>1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin. <br> | <p>1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin. <br> | ||
2. Inoculation of pACYC177+OmpA_alpha and pACYC177+OmpA_omega - liquid LB with kanamycin.</p> | 2. Inoculation of pACYC177+OmpA_alpha and pACYC177+OmpA_omega - liquid LB with kanamycin.</p> | ||
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| + | Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr | ||
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| + | Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50 | ||
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| + | wieczorem mieżenie OD : tabelka | ||
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1. Separete transformant colonies (tranformation from previous day) inoculated to liquid LB with kanamycin.
Sprawdzanie czy omp_omega_A_alfa daje oporność na AP Piotr Zaszczepienie omp_omega_A_alfa z rużnych stężeń IPTG: 0; 0,1 ; 0,25 ; 0,5; 0,75; 1 do takich samych stężeń IPTG, ale z rużnymi stężeniami AP (25;50;75;100) w stosunku 1:50 wieczorem mieżenie OD : tabelka
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