Team:Warsaw/Calendar-Main/22 July 2008

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<p>'''Cloning of protein A DNA to OmpA constructs''' <br>
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<h3>Cloning of protein A DNA to OmpA constructs</h3>
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1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega). <br>
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<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation of plasmids</a> from cultures inocluated on previous day (pACYC177+OmpA_A_omega).</li>
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2. Control digest of isolated plasmids with BamHI and SacI . </p>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of isolated plasmids with BamHI and SacI. </li></ol>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
<h3>Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA<br>
Paweł</h3>
Paweł</h3>
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<p><ol><li>Results of ligation: 6 colonies grown.</li>
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<ol><li>Results of ligation: 6 colonies grown.</li>
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<li>Each colony cultured overnight in LB + ampicillin</li></ol></p>
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<li>Each colony cultured overnight in LB + ampicillin</li></ol>
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Revision as of 17:11, 5 October 2008

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Cloning of protein A DNA to OmpA constructs

  1. Isolation of plasmids from cultures inocluated on previous day (pACYC177+OmpA_A_omega).
  2. Control digest of isolated plasmids with BamHI and SacI.

Cloning of protein Z DNA to pET15b-OmpA-omega in place of OmpA
Paweł

  1. Results of ligation: 6 colonies grown.
  2. Each colony cultured overnight in LB + ampicillin

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