Team:Warsaw/Calendar-Main/14 July 2008

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<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment and Z DNA fragment isolated on 10 July. </li>
<a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of isolated DNA fragment and Z DNA fragment isolated on 10 July. </li>
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<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top">TOP10</a> strain with ligation. </li>
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<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation. </li>
<li> Transformants plating on LB + kanamycin. </li>
<li> Transformants plating on LB + kanamycin. </li>
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Revision as of 16:47, 5 October 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
  2. Gel eloctrophoresis and gel-out (4300 bp band).
  3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
  4. Transformation of E. coli TOP10 strain with ligation.
  5. Transformants plating on LB + kanamycin.



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