Team:Warsaw/Calendar-Main/15 July 2008

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<h3> Preparation of alfa+A conctruct<br>Antoni</h3>
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<li>gel electrophoresis
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Revision as of 15:43, 9 October 2008

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Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. 4 colonies from transformation pACYC177+OmpA_Z_alpha were inoculated to liquid LB broth with kanamycin



Preparation of alfa+A conctruct
Antoni

  1. Gradient PCR on alpha+A
  2. PCR with gradient of DMSO on alpha+A
  3. gel electrophoresis


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