Team:Warsaw/Calendar-Main/10 June 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
-
<html><h3>Michał L., Ewa, Marcin</h3>
+
<html><h4>Piotr:</h4>
-
<ol><li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Plasmid isolation</a> from overnight culture of our transformants.</li>
+
<p>Primers for fusion of protein A, OmpA, alpha and omega fragments of beta-lactamase were designed:</p>
-
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and SalI.</li>
 
-
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + AID transcription fusion with AID-T7 RNA-polymerase translation fusion with HindIII and EcoRI.</li>
+
<p><a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL_link">alphaL+link14+homo (alphaL_link)</a>
 +
<br>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaP_XB">alphaP+STOP+XbaI+BamHI (alphaP_XB)</a><br>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">omegaL+link14+homo (omegaL_link)</a><br>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)</a><br>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaL_N">OmpAL+NdeI (ompaL_N)</a> <br>
 +
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmpaP_link">OmpAP+link8+SacI+NotI+homo (ompaP_link)</p><br>
 +
</html>
-
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + T7 RNA-polymerase with EcoRI.</li>
 
-
 
-
<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of pMPM-T5 + T7 RNA-polymerase with BamHI and XhoI.</li>
 
-
 
-
<li>DNA gel electrophoresis of digest products.</li>
 
-
 
-
<li>Picking out proper colonies of transformants.</li>
 
-
</ol>
 
</html>
</html>

Revision as of 19:18, 5 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
next notebook entry

 


Piotr:

Primers for fusion of protein A, OmpA, alpha and omega fragments of beta-lactamase were designed:

alphaL+link14+homo (alphaL_link)
alphaP+STOP+XbaI+BamHI (alphaP_XB)
omegaL+link14+homo (omegaL_link)
omegaP+STOP+EcoRI+PstI+BamHI (omegaP_EPB)
OmpAL+NdeI (ompaL_N)
OmpAP+link8+SacI+NotI+homo (ompaP_link)


</html>