Team:Warsaw/Calendar-Main/26 May 2008

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<html><h3>Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7</h3>
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<h4>Michał K.:</h4>
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Michał K.</h3>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008">15 May</a> (p.1 and p.4).</li>
<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of purified products from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/15_May_2008">15 May</a> (p.1 and p.4).</li>

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Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7
Michał K.

  1. Ligation of purified products from 15 May (p.1 and p.4).
  2. Electroporation of E. coli TOP10 with ligation product.
  3. Ligation of digested products from 15 May (p.4 and p.5).
  4. Electroporation of E. coli TOP10 with ligation product.
  5. Transformants plating on LB + tetracycline.

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