Team:Warsaw/Calendar-Main/14 July 2008

From 2008.igem.org

(Difference between revisions)
Line 16: Line 16:
<br>
<br>
<br>
<br>
 +
 +
<h3> Preparation of alfa+A conctruct<br>Antoni</h3>
 +
<p><ol><li>Protein A PCR on pKS+A</li>
 +
<li>PCR on alpha</li>
 +
<li>Gel electrophoresis</li>
 +
<li>Gel-out</li>
 +
<li>Digest with
 +
</li>
 +

Revision as of 15:37, 9 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein Z DNA to OmpA constructs
Michał K.

  1. Digest and dephosphorylation (with CIAP) of pACYC177+OmpA_alpha with SacI and NotI.
  2. Gel eloctrophoresis and gel-out (4300 bp band).
  3. Ligation of isolated DNA fragment and Z DNA fragment isolated on 10 July.
  4. Transformation of E. coli TOP10 strain with ligation.
  5. Transformants plating on LB + kanamycin.



    Preparation of alfa+A conctruct
    Antoni

    1. Protein A PCR on pKS+A
    2. PCR on alpha
    3. Gel electrophoresis
    4. Gel-out
    5. Digest with

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/14_July_2008"