Team:Warsaw/Calendar-Main/5 September 2008

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<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p>
<li>In case of A-alpha: pellet discarded, supernatant kept for further purification steps</li></ol></p>
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<h3>Protein A mutagenesis<br>Paweł</h3>
 
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<p><ol><li>Treatment of mutageneses as on 2nd September. </li></ol></p>
 
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Revision as of 15:24, 9 October 2008

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Purification of proteins: A-alpha, Z-alpha and Z-omega
Piotr

  1. Bacteria centrifuged and sonicated (according to protocol).
  2. Sonicate centrifuged.
  3. In case of Z-alpha and Z-omega: supernatant discarded, pellet resuspended in ddH20, centrifuged and again resuspended in ddH20
  4. In case of A-alpha: pellet discarded, supernatant kept for further purification steps

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